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ID 112928
タイトル別表記
ナローバンドUVBがHeLa細胞とアレルギー性鼻炎モデルラットの鼻粘膜におけるヒスタミンH1受容体遺伝子発現の亢進とアポトーシスの誘導に与える影響
著者
藤井, 達也 徳島大学大学院医科学教育部(医学専攻) KAKEN研究者をさがす
Okamoto, Kentaro Tokushima University
Sanada, Nanae Tokushima University
Yamada, Takuya Tokushima University
Sugiyama, Manabu Tokushima University
Michinaga, Shotaro Osaka Ohtani University
Kitayama, Mika Tokushima University
キーワード
Allergic rhinitis
apoptosis
histamine H1 receptor gene
narrowband-ultraviolet B
phototherapy
資料タイプ
学位論文
抄録
Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12- myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and upregulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.
掲載誌名
Journal of Pharmacological Sciences
ISSN
13478613
cat書誌ID
AA12022662
出版者
Elsevier|Japanese Pharmacological Society
138
1
開始ページ
54
終了ページ
62
発行日
2018-09-10
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Tatsuya Fujiiの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3246号
学位記番号
甲医第1395号
学位授与年月日
2018-11-22
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系
薬学系