イイダ, シンヤ Department of Virology, The University of Tokushima School of Medicine
オオシマ, ヨウコ Department of Virology, The University of Tokushima School of Medicine
コヤマ, ハジメ Department of Virology, The University of Tokushima School of Medicine
A mutant designated NL-E65, which lacks the expression of entire vpu gene, was constructed from T-cell tropic wild-type (wt) human immunodeficiency virus type1(HIV-1) clone and monitored for its replication property in human cells, along with a mutant NL-Ss which expresses a C-terminal truncated Vpu. The mutant NL-Ss could grow in two cell lines and in all peripheral blood mononuclear cell (PBMC) preparations to some extent, with kinetics similar to those of wt virus. Likewise, the mutant NL-E65exhibited a replication property typical to the vpu mutant in the two cell lines and in all PBMC cultures, growing at a low level. Along with the results previously reported, these data indicate that HIV-1 Vpu is dispensable for virus replication in any of the types of cells so far tested.
The journal of medical investigation : JMI
LID201111161006.pdf 162 KB