Tanaka, Yu NIH
近藤, 健太 NIH
Kondo, Hiroyuki University of Tokushima
Palin, Amy C. NIH
Hoffmann, Victoria NIH
Uda, Shinsuke Kyushu University
Motosugi, Ryo The University of Tokyo
Hamazaki, Jun The University of Tokyo
Kubota, Hiroyuki Kyushu University
Murata, Shigeo The University of Tokyo
Tanaka, Keiji Tokyo Metropolitan Institute for Medical Science
The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology.
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
celrep_29_9_2901.pdf 5.28 MB