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タイトル別表記
Rac1シグナル伝達経路を介したカンピロバクター・ジェジュニの宿主細胞侵入部位へのLC3動員
New Function of Autophagy in C. jejuni Invasion
著者
福島, 志帆 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
下畑, 隆明 Tokushima University|Fukui Prefectural University KAKEN研究者をさがす
Inoue, Yuri Tokushima University
Kido, Junko Tokushima University
キーワード
Campylobacter jejuni
autophagy
LC3
Rac1
invasion
資料タイプ
学位論文
抄録
Campylobacter jejuni is a leading cause of food-borne disease worldwide. The pathogenicity of C. jejuni is closely associated with the internalization process in host epithelial cells, which is related to a host immune response. Autophagy indicates a key role in the innate immune system of the host to exclude invasive pathogens. Most bacteria are captured by autophagosomes and degraded by autophagosome-lysosome fusion in host cells. However, several pathogens, such as Salmonella and Shigella, avoid and/or escape autophagic degradation to establish infection. But autophagy involvement as a host immune response to C. jejuni infection has not been clarified. This study revealed autophagy association in C. jejuni infection. During infection, C. jejuni activated the Rho family small GTPase Rac1 signaling pathway, which modulates actin remodeling and promotes the internalization of this pathogen. In this study, we found the LC3 contribution to C. jejuni invasion signaling via the Rac1 signaling pathway. Interestingly, during C. jejuni invasion, LC3 was recruited to bacterial entry site depending on Rac1 GTPase activation just at the early step of the infection. C. jejuni infection induced LC3-II conversion, and autophagy induction facilitated C. jejuni internalization. Also, autophagy inhibition attenuated C. jejuni invasion step. Moreover, Rac1 recruited LC3 to the cellular membrane, activating the invasion of C. jejuni. Altogether, our findings provide insights into the new function of LC3 in bacterial invasion. We found the interaction between the Rho family small GTPase, Rac1, and autophagy-associated protein, LC3.
掲載誌名
Frontiers in Cellular and Infection Microbiology
ISSN
22352988
出版者
Frontiers Media S.A.
12
開始ページ
829682
発行日
2022-03-03
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shiho Fukushimaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3601号
学位記番号
甲栄第289号
学位授与年月日
2022-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系