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ID 115427
タイトル別表記
Pyrogallol inhibits NFAT signal
著者
Nakano, Tomohiro Tokushima University
Ikeda, Mitsuhiro Tokushima University
Wakugawa, Tomoharu Tokushima University
Kaminuma, Osamu Hiroshima University
Kitamura, Noriko The Tokyo Metropolitan Institute of Medical Science
福井, 裕行 Medical Corporation Kinshukai|Osaka Ohtani University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
allergic rhinitis
calcineurin
histamine H1 receptor gene
Interleukin-9 gene
nuclear factor of activated T-cells
資料タイプ
学術雑誌論文
抄録
As the expression level of allergic disease sensitive genes are correlated with the severity of allergic symptoms, suppression of these gene expressions could be promising therapeutics. We demonstrated that protein kinase Cδ / heat shock protein 90-mediated H1R gene expression signaling and nuclear factor of activated T-cells (NFAT)-mediated IL-9 gene expression signaling are responsible for the pathogenesis of pollinosis. Treatment with Awa-tea combined with wild grape hot water extract suppressed these signaling and alleviated nasal symptoms in toluene-2,4-diisocyanate (TDI)-sensitized rats. However, the underlying mechanism of its anti-allergic activity is not elucidated yet. Here, we sought to identify an anti-allergic compound from Awa-tea and pyrogallol was identified as an active compound. Pyrogallol strongly suppressed ionomycin-induced up-regulation of IL-9 gene expression in RBL-2H3 cells. Treatment with pyrogallol in combination with epinastine alleviated nasal symptoms and suppressed up-regulation of IL-9 gene expression in TDI-sensitized rats. Pyrogallol itself did not inhibit calcineurin phosphatase activity. However, pyrogallol suppressed ionomycin-induced dephosphorylation and nuclear translocation of NFAT. These data suggest pyrogallol is an anti-allergic compound in Awa-tea and it suppressed NFAT-mediated IL-9 gene expression through the inhibition of dephosphorylation of NFAT. This might be the underlying mechanism of the therapeutic effects of combined therapy of pyrogallol with antihistamine.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
出版者
Tokushima University Faculty of Medicine
67
3-4
開始ページ
289
終了ページ
297
並び順
289
発行日
2020-08
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
薬学系
医学系