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ID 113338
タイトル別表記
レスベラトロールは子宮肉腫細胞において、wntシグナルを抑制することによりアポトーシスを誘導し、細胞増殖を抑制する
著者
峯田, あゆか 徳島大学大学院医科学教育部(医学専攻)
炬口, 恵理 Tokushima University
キーワード
resveratrol
uterine sarcoma
Wnt signaling pathway
β-catenin
glycogen synthase kinase-3β
資料タイプ
学位論文
抄録
Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.
掲載誌名
Experimental and Therapeutic Medicine
ISSN
17920981
17921015
cat書誌ID
AA12610820
出版者
Spandidos Publications
17
3
開始ページ
2242
終了ページ
2246
発行日
2019-01-28
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者AYUKA MINEDAの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© Mineda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.( https://creativecommons.org/licenses/by-nc-nd/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3254号
学位記番号
甲医第1403号
学位授与年月日
2019-03-22
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系