直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 111886
著者
Yoshida, Ichiro Shikoku Junior College
Ito, Chihiro Tokushima University
Matsuda, Shinya Tokushima University
Yanaka, Noriyuki Hiroshima University
キーワード
melanogenesis
alisol B
Kampo
tyrosinase
microphthalmia-associated transcription factor (MITF)
資料タイプ
学術雑誌論文
抄録
To develop new whitening agents from natural products, we screened 80 compounds derived from crude drugs in Kampo medicine in a melanin synthesis inhibition assay using murine B16 melanoma cells. The screen revealed that treatment with alisol B, a triterpene from Alismatis rhizoma, significantly decreased both melanin content and cellular tyrosinase activity in B16 cells. However, alisol B did not directly inhibit mushroom tyrosinase activity in vitro. Therefore, we investigated the mechanism underlying the inhibitory effect of alisol B on melanogenesis. Alisol B suppressed mRNA induction of tyrosinase and its transcription factor, microphthalmia-associated transcription factor (MITF). Furthermore, alisol B reduced the phosphorylation of CREB and maintained the activation of ERK1/2. These results suggest that the reduction in melanin production by alisol B is due to the downregulation of MITF through the suppression of CREB and activation of ERK, and that alisol B may be useful as a new whitening agent.
掲載誌名
Bioscience, Biotechnology, and Biochemistry
ISSN
09168451
13476947
cat書誌ID
AA10824164
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
81
3
開始ページ
534
終了ページ
540
発行日
2017-01-04
備考
This is an electronic version of an article published in Bioscience, Biotechnology, and Biochemistry 81, 3, 534-540, 2017. Bioscience, Biotechnology, and Biochemistry is available online at: www.tandfonline.com/10.1080/09168451.2016.1268042.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系