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ID 110419
著者
ニワ, エイコ Faculty of Integrated Arts and Sciences, Tokushima University
ミタニ, ツヨシ Faculty of Integrated Arts and Sciences, Tokushima University
サイトウ, ショウヘイ Faculty of Integrated Arts and Sciences, Tokushima University
金丸, 芳 Faculty of Bioscience and Bio-industry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
イシダ, シロウ Faculty of Pharmaceutical Sciences, Tokushima Bunri University
横井川, 久己男 Faculty of Bioscience and Bio-industry, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
小山, 保夫 Faculty of Bioscience and Bio-industry, Tokushima University KAKEN研究者をさがす
キーワード
arachidonic acid
cytotoxicity
lymphocyte
oxidative stress
zinc
資料タイプ
学術雑誌論文
抄録
Previous studies on the cytotoxicity of arachidonic acid (ARA) elucidated the involvement of oxidative stress and Ca2+. In the present study, the Zn2+-related cytotoxicity of ARA was studied by a flow cytometric technique with appropriate fluorescent probes in rat thymocytes. Addition of 10 μM ZnCl2 enhanced the increase in cell lethality induced by 10 μM ARA. The removal of Zn2+ by Zn2+ chelators attenuated the ARA-induced increase in cell lethality. Thus, Zn2+ is suggested to be involved in ARA cytotoxicity. ARA at 3–10 μM elevated intracellular Zn2+ level. The Zn2+ chelators attenuated the ARA-induced increase in intracellular Zn2+ level while ARA significantly increased intracellular Zn2+ level in the presence of 3 μM ZnCl2, suggesting the involvement of external Zn2+. Zn2+ reportedly exerts cytotoxic action under oxidative stress induced by hydrogen peroxide, via an excessive increase in intracellular Zn2+ levels. Since ARA induces oxidative stress, the simultaneous administration of zinc and ARA may be harmful.
掲載誌名
Food and Chemical Toxicology
ISSN
02786915
cat書誌ID
AA10627174
AA11526376
出版者
Elsevier
96
開始ページ
177
終了ページ
182
並び順
177
発行日
2016-08-08
備考
Copyright © 2016 Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系