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ID 115112
タイトル別表記
Cerebral Glycogen Distribution and Aging
著者
Oe, Yuki RIKEN Brain Science Institute
Ashida, Hitoshi Kobe University
Nakamura, Kouichi C. Kyoto University
Hirase, Hajime RIKEN Brain Science Institute|Saitama University
キーワード
astrocytes
focused microwave irradiation
glycogen
immunohistochemistry
aging
資料タイプ
学術雑誌論文
抄録
In the brain, glycogen metabolism has been implied in synaptic plasticity and learning, yet the distribution of this molecule has not been fully described. We investigated cerebral glycogen of the mouse by immunohistochemistry (IHC) using two monoclonal antibodies that have different affinities depending on the glycogen size. The use of focused microwave irradiation yielded well‐defined glycogen immunoreactive signals compared with the conventional periodic acid‐Schiff method. The IHC signals displayed a punctate distribution localized predominantly in astrocytic processes. Glycogen immunoreactivity (IR) was high in the hippocampus, striatum, cortex, and cerebellar molecular layer, whereas it was low in the white matter and most of the subcortical structures. Additionally, glycogen distribution in the hippocampal CA3‐CA1 and striatum had a ‘patchy’ appearance with glycogen‐rich and glycogen‐poor astrocytes appearing in alternation. The glycogen patches were more evident with large‐molecule glycogen in young adult mice but they were hardly observable in aged mice (1–2 years old). Our results reveal brain region‐dependent glycogen accumulation and possibly metabolic heterogeneity of astrocytes.
掲載誌名
GLIA
ISSN
10981136
cat書誌ID
AA10687234
出版者
Wiley
64
9
開始ページ
1532
終了ページ
1545
発行日
2016-06-29
権利情報
This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系