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ID 109370
タイトル別表記
Cyclopamineは大腸癌癌幹細胞のソニック・ヘッジホッグとその下流遺伝子発現を抑制する
Sonic Hedgehog Downstream Genes in Cancer Stem Cells
著者
バトサイハン, バトエルデネ 徳島大学大学院医科学教育部(医学専攻)
栗田, 信浩 The University of Tokushima KAKEN研究者をさがす
キーワード
Cancer stem-like cells
epithelial-mesenchymal transition
cancer cells
sonic hedgehog
資料タイプ
学位論文
抄録
Backround: Most solid cancers including colon cancer are believed to be initiated from and maintained by cancer stem cells (CSCs), that are responsible for treatment resistance, resulting in tumor relapse. The aim of this study was to clarify the possible role of the Sonic Hedgehog (Shh) signaling pathway in the regulation of cancer stem cells. Materials and Methods: The HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Gene expressions were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) from cells treated with and without cyclopamine. Results: HCT-116 sphere-derived cells grown in serum-free, non-adherent culture, showed significantly increased expression of stem cell markers, Shh downstream genes and epithelial-mesenchymal transition (EMT) markers compared to parental cells grown in conventional culture. The expression of stemness markers, Shh downstream genes and EMT markers were higher in cancer spheres than the parental cell line and down-regulated by cyclopamine treatment in a dose-dependent manner. Conclusion: Overall, these findings show that cyclopamine treatment could down-regulate the expression of stemness markers, shh downstream genes and EMT markers on HCT-116 spheres.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
34
11
開始ページ
6339
終了ページ
6344
発行日
2014-11-03
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201505251001.pdf
論文本文 : k2796_fulltext.pdf
本論文は,著者BAT-ERDENE BATSAIKHANの学位論文として提出され, 学位審査・授与の対象となっている。
著者の申請により要約(2015-05-25公開)に替えて論文全文を公開(2022-12-14)
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2796号
学位記番号
甲医第1238号
学位授与年月日
2015-03-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
教養教育院
医学系