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ID 113051
著者
Nakamura, Toru The University of Tokyo|Hokkaido University
片桐, 豊雅 The University of Tokyo|The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Sato, Shoki Hokkaido University
Kushibiki, Toshihiro Hokkaido University
Hontani, Koji Hokkaido University
Tsuchikawa, Takahiro Hokkaido University
Hirano, Satoshi Hokkaido University
Nakamura, Yusuke The University of Tokyo|The University of Chicago
キーワード
pancreatic cancer
novel gene
molecular target
資料タイプ
学術雑誌論文
抄録
Clinical outcome of pancreatic ductal adenocarcinoma (PDAC) has not been improved in the last three decades due to the lack of effective molecular-targeted drugs. To identify a novel therapeutic target for PDAC, we have performed genome-wide anamysis and found that Homo sapiens chromosome 16 open reading frame 74 (C16orf74) was up-regulated in the vast majority of PDAC. Overexpression of C16orf74 protein detected by immunohistochemical analysis was an independent prognostic factor for patients with PDAC. The knockdown of endogenous C16orf74 expression in the PDAC cell lines KLM-1 and PK-59 by vector-based small hairpin-RNA (shRNA) drastically attenuated the growth of those cells, whereas ectopic C16orf74 overexpression in HEK293T and NIH3T3 cells promoted cell growth and invasion, respectively. More importantly, the endogenous threonine 44 (T44)-phosphorylated form of C16orf74 interacted with the protein phosphatase 3 catalytic subunit alpha (PPP3CA) via the PDIIIT sequence in the PPP3CA-binding motif within the middle portion of C16orf74 in PDAC cells. The overexpression of mutants of C16orf74 lacking the PDIIIT sequence or T44 phosphorylation resulted in the suppression of invasive activity compared with wild-type C16orf74, indicating that their interaction should be indispensable for PDAC cell invasion. These results suggest that C16orf74 plays an important role for PDAC invasion and proliferation, and is a promising target for a specific treatment for patients with PDAC.
掲載誌名
Oncotarget
ISSN
19492553
出版者
Impact Journals, LLC
8
31
開始ページ
50460
終了ページ
50475
発行日
2016-07-28
権利情報
Copyright: Nakamura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0)(https://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所