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ID 110115
タイトル別表記
生後早期のラットでは免疫ストレスによるLH分泌抑制反応が確立されていない
著者
ムンフザヤ, ムンフサイハン 徳島大学大学院医科学教育部(医学専攻)
松崎, 利也 The University of Tokushima KAKEN研究者をさがす
Tungalagsuvd, Altankhuu The University of Tokushima
Kawami, Takako The University of Tokushima
桑原, 章 The University of Tokushima KAKEN研究者をさがす
キーワード
LPS
Cytokines
Stress
Hyporesponsive period
HPG axis
LH
資料タイプ
学位論文
抄録
Some physiological functions display weak responses to stress in the early neonatal period; i.e., theyexhibit stress hyporesponse periods. In this study, we evaluated whether gonadotropin regulatory fac-tors exhibit stress hyporesponsive periods in male and female rats. Rats were intraperitoneally injectedwith lipopolysaccharide (100 μg/kg) (LPS group) or saline (control group) on postnatal day (PND) 5,10, 15, or 25. Then, their serum luteinizing hormone (LH) concentrations and hypothalamic mRNAlevels of gonadotropin regulatory factors; i.e., kisspeptin (Kiss1), the kisspeptin receptor (Kiss1r), andgonadotropin-releasing hormone (GnRH), were measured at 2 h after the injection. The hypothalamicmRNA levels of pro-inflammatory cytokines were also measured because they suppress gonadotropinsecretion. The serum LH concentration of the LPS group was lower than that of the control group atPND25 in both sexes, but no such difference was seen at PND5, 10, or 15 in either sex. In both sexes,the hypothalamic tumor necrosis factor (TNF)α and interleukin (IL)-6 mRNA expression levels of the LPSgroup were higher than those of the control group at PND25, but not at PND5 or 10. The hypothalamicIL-1β mRNA expression level of the LPS group was higher than that of the control group at all time points. The hypothalamic Kiss1, Kiss1r, and GnRH mRNA expression levels of the LPS and control groups did notdiffer at any time point in either sex. These findings suggest that gonadotropin regulatory factors exhibitstress hyporesponse periods. The hypothalamic–pituitary–gonadal axis (HPG) might become responsiveto immune stress between PND15 and 25, which could be related to enhanced hypothalamic cytokineexpression. The avoidance of infectious stress during the early neonatal period might be important fornormal development of the HPG axis.
掲載誌名
International Journal of Developmental Neuroscience
ISSN
07365748
cat書誌ID
AA10637655
AA11531026
出版者
Elsevier|International Society for Developmental Neuroscience
46
開始ページ
38
終了ページ
43
発行日
2015-07-15
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨:LID201705191010.pdf
論文本文:k3023_fulltext.pdf
本論文は, 著者Munkhsaikhan Munkhzayaの学位論文として提出され, 学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3023号
学位記番号
甲医第1327号
学位授与年月日
2017-03-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院