直近一年間の累計
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ID 116259
著者
Maeda, Ryo Osaka University
Kitano, Satsuki Kyoto University
Miyachi, Hitoshi Kyoto University
Fukuda, Mikiko Kyoto University
Shinkai, Yoichi RIKEN
立花, 誠 Osaka University|Tokushima University KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
Histone H3 lysine 9 (H3K9) methylation is dynamically regulated by methyltransferases and demethylases. In spermatogenesis, prospermatogonia differentiate into differentiating or undifferentiated spermatogonia after birth. However, the epigenetic regulation of prospermatogonia to spermatogonia transition is largely unknown. We found that perinatal prospermatogonia have extremely low levels of di-methylated H3K9 (H3K9me2) and that H3K9 demethylases, JMJD1A and JMJD1B, catalyze H3K9me2 demethylation in perinatal prospermatogonia. Depletion of JMJD1A and JMJD1B in the embryonic germline resulted in complete loss of male germ cells after puberty, indicating that H3K9me2 demethylation is essential for male germline maintenance. JMJD1A/JMJD1B-depleted germ cells were unable to differentiate into functional spermatogonia. JMJD1 isozymes contributed to activation of several spermatogonial stem cell maintenance genes through H3K9 demethylation during the prospermatogonia to spermatogonia transition, which we propose is key for spermatogonia development. In summary, JMJD1A/JMJD1B-mediated H3K9me2 demethylation promotes prospermatogonia to differentiate into functional spermatogonia by establishing proper gene expression profiles.
掲載誌名
Stem Cell Reports
ISSN
22136711
出版者
International Society for Stem Cell Research|Cell Press
15
2
開始ページ
424
終了ページ
438
発行日
2020-07-16
権利情報
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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言語
eng
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出版社版
部局
先端酵素学研究所
技術支援部