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ID 115825
タイトル別表記
Molecular analysis for bacterial contamination
著者
Kokeguchi, Susumu Okayama University
キーワード
Bacterial contamination
Dental unit water line
Molecular analysis
PCR-DGGE
資料タイプ
学術雑誌論文
抄録
Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.
掲載誌名
New Microbiologica
ISSN
11217138
cat書誌ID
AA11980981
出版者
EDIMES
39
2
開始ページ
143
終了ページ
145
発行日
2016-04
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系