アクセス数 : ?
ダウンロード数 : ?
ID 116300
タイトル別表記
Leukocyte and lymphoid organ ontogeny
著者
Ferreirinha, Pedro Universidade do Porto
Ribeiro, Camila Universidade do Porto
Landry, Jonathan J. M. European Molecular Biology Laboratory
Meireles, Catarina Universidade do Porto
White, Andrea J. University of Birmingham
Araújo, Leonor Universidade do Porto
Benes, Vladimir European Molecular Biology Laboratory
Anderson, Graham University of Birmingham
Alves, Nuno L. Universidade do Porto
キーワード
autoimmune regulator
differentiation
medullary thymic epithelial cell
thymus
tolerance induction
資料タイプ
学術雑誌論文
抄録
Autoimmune regulator+ (Aire) medullary thymic epithelial cells (mTECs) play a critical role in tolerance induction. Several studies demonstrated that Aire+mTECs differentiate further into Post-Aire cells. Yet, the identification of terminal stages of mTEC maturation depends on unique fate-mapping mouse models. Herein, we resolve this limitation by segmenting the mTEChi(MHCIIhiCD80hi) compartment into mTECA/hi (CD24−Sca1−), mTECB/hi (CD24+Sca1−), and mTECC/hi (CD24+Sca1+). While mTECA/hi included mostly Aire-expressing cells, mTECB/hi contained Aire+ and Aire− cells and mTECC/hi were mainly composed of cells lacking Aire. The differential expression pattern of Aire led us to investigate the precursor-product relationship between these subsets. Strikingly, transcriptomic analysis of mTECA/hi, mTECB/hi, and mTECC/hi sequentially mirrored the specific genetic program of Early-, Late- and Post-Aire mTECs. Corroborating their Post-Aire nature, mTECC/hi downregulated the expression of tissue-restricted antigens, acquired traits of differentiated keratinocytes, and were absent in Aire-deficient mice. Collectively, our findings reveal a new and simple blueprint to survey late stages of mTEC differentiation.
掲載誌名
European Journal of Immunology
ISSN
15214141
出版者
Wiley|European Federation of Immunological Societies
51
2
開始ページ
311
終了ページ
318
発行日
2020-08-26
権利情報
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License(https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所
医学系