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ID 106279
タイトル別表記
Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響
Effect of eDNA on biofilm formation
著者
アシキン, ヌル 徳島大学大学院口腔科学教育部(口腔科学専攻)
弘田, 克彦 The University of Tokushima KAKEN研究者をさがす
平尾, 功治 The University of Tokushima KAKEN研究者をさがす
Liu, Dali Shanghai Jiao Tong University
高橋, 加奈子 The University of Tokushima KAKEN研究者をさがす
Shu, Rong Shanghai Jiao Tong University
キーワード
extracellular DNA
extracellular HLP
biofilm development
Streptococcus intermedius
DNA concentration
資料タイプ
学位論文
抄録
Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.
Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains.
Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.
Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
掲載誌名
Journal of Applied Microbiology
ISSN
13652672
13645072
cat書誌ID
AA11115564
出版者
The Society for Applied Microbiology|John Wiley & Sons Inc
115
1
開始ページ
260
終了ページ
270
発行日
2013-03-29
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201409191007.pdf
審査要旨 : LID201409191008.pdf
論文本文 : k2757_fulltext.pdf
本論文は,著者Asikin Nurの学位論文として提出され,学位審査・授与の対象となっている。
著者の申請により要約(2014-09-20公開)に替えて論文全文を公開(2019-10-09)
権利情報
This is the peer reviewed version of the following article: Nur, A., Hirota, K., Yumoto, H., Hirao, K., Liu, D., Takahashi, K., Murakami, K., Matsuo, T., Shu, R. and Miyake, Y. (2013), Effects of extracellular DNA and DNA‐binding protein on the development of a Streptococcus intermedius biofilm. J Appl Microbiol, 115: 260-270., which has been published in final form at https://doi.org/10.1111/jam.12202. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2757号
学位記番号
甲口第386号
学位授与年月日
2014-09-10
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
病院