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ID 117783
タイトル別表記
An Improved DDS Tool with Versatile Cell-targeting Ability
著者
Ohkubo, Yukimasa University of Tokushima
Tamura, Masato University of Tokushima
大倉, 一人 Suzuka University
キーワード
Cholesterol-dependent cytolysin
drug delivery system
Z-domain
chimera protein
資料タイプ
学術雑誌論文
抄録
Background/Aim: The aim of this study was to develop an improved drug delivery system (DDS) tool with enhanced versatility in the cell-targeting step using as Z-domain, a modified IgG binding domain of protein A from Staphylococcus aureus, as an IgG adapter domain. Materials and Methods: The chimera protein expression system composed of the Z-domain and chimeric cholesterol-dependent cytolysin mutant named His-Z-CDC(ss)IS was constructed in Escherichia coli. His-Z-CDC(ss)IS was purified by Ni-affinity chromatography, and its abilities for controlled pore formation, membrane binding, IgG binding, and target cell-specific delivery of liposomes carrying medicine were investigated. Results and Discussion: His-Z-CDC(ss)IS purified by Ni-affinity chromatography indicated pore-forming activity only under disulfide bond reducing conditions. His-Z-CDC(ss)IS also demonstrated an ability to bind both IgG and cholesterol-embedded liposomes via its Z-domain and domain 4, respectively. Furthermore, anticarcinoembryonic antigen (CEA) IgG-bound His-Z-CDC(ss)IS indicated effective delivery of liposomes carrying drugs to CEA-expressing cells. Conclusion: His-Z-CDC(ss)IS was revealed to be an improved DDS tool with enhanced versatility in cell targeting.
掲載誌名
Anticancer Research
ISSN
02507005
17917530
cat書誌ID
AA10625860
AA12440673
出版者
The International Institute of Anticancer Research
33
7
開始ページ
2905
終了ページ
2910
発行日
2013-06-18
EDB ID
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系