樋浦, 明夫 Oral Histology, School of Dentistry, University of Tokushima KAKEN研究者をさがす
中川, 弘 Pediatric Dentistry, Tokushima University Hospital, University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
The aim of this study was to examine whether the neonatal injection of capsaicin induces DNA ladder formation, as an indicator of apoptosis, in the rat trigeminal ganglion (TG). The DNA extracted from the TGs after treatment with capsaicin (50 mg/kg) at 2 days of age exhibited diffuse DNA fragmentation instead of “ladder formation”, as determined by agarose gel electrophoresis. In contrast, clear DNA ladder formation was induced in human osteosarcoma cells treated with okadaic acid (OA) at concentrations of 10 and 20 nM. These results suggest that capsaicin cannot induce apoptosis of TG neurons sufficient for detectable DNA ladder formation. Thus, it is conceivable that capsaicin mainly causes necrosis rather than apoptosis in rodent primary sensory neurons.
Article URL: http://www.webmedcentral.com/article_view/3708
This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
LID201211061001.pdf 164 KB