Lymph Node Stromal Cell Subsets
Takeuchi, Arata Niigata University
Ozawa, Madoka Niigata University
Kanda, Yasuhiro Niigata University
Kozai, Mina University of Tokushima
Kurosawa, Yoichi Niigata University
Rahman, Md Azizur Niigata University
Kawamura, Toshihiko Niigata University|Kitasato University
Shichida, Yuto Niigata University
Umemoto, Eiji Osaka University
Miyasaka, Masayuki University of Turku|Osaka University
Ludewig, Burkhard Kantonal Hospital St. Gallen
Nagasawa, Takashi Osaka University
Katakai, Tomoya Niigata University
fibroblastic stromal cells
The spatiotemporal regulation of immune responses in the lymph node (LN) depends on its sophisticated tissue architecture, consisting of several subcompartments supported by distinct fibroblastic stromal cells (FSCs). However, the intricate details of stromal structures and associated FSC subsets are not fully understood. Using several gene reporter mice, we sought to discover unrecognized stromal structures and FSCs in the LN. The four previously identified FSC subsets in the cortex are clearly distinguished by the expression pattern of reporters including PDGFRb, CCL21-ser, and CXCL12. Herein, we identified a unique FSC subset expressing both CCL21-ser and CXCL12 in the deep cortex periphery (DCP) that is characterized by preferential B cell localization. This subset was clearly different fromCXCL12highLepRhigh FSCs in themedullary cord, which harbors plasma cells. B cell localization in the DCP was controlled chiefly by CCL21-ser and, to a lesser extent, CXCL12. Moreover, the optimal development of the DCP as well as medulla requires B cells. Together, our findings suggest the presence of a unique microenvironment in the cortex-medulla boundary and offer an advanced view of the multi-layered stromal framework constructed by distinct FSC subsets in the LN.
Frontiers in Immunology
Frontiers Media S.A.
Copyright © 2018 Takeuchi, Ozawa, Kanda, Kozai, Ohigashi, Kurosawa, Rahman, Kawamura, Shichida, Umemoto, Miyasaka, Ludewig, Takahama, Nagasawa and Katakai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (https://creativecommons.org/licenses/by/4.0/) The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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