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ID 118778
タイトル別表記
OTUB1-YAP1経路の頭頸部扁平上皮癌の悪性化への関与
著者
キン, セイケン 徳島大学大学院口腔科学研究科(口腔科学専攻)
Hagita, Hiroko Tokushima University
Sarubo, Motoharu Tokushima University
Qi, Guangying Guilin Medical University
キーワード
Deubiquitinating enzyme (DUB)
head and neck squamous cell carcinoma
invasion
OTUB1
YAP
資料タイプ
学位論文
抄録
Background: Comprehending the molecular mechanisms underlying head and neck squamous cell carcinoma (HNSCC) is vital for the development of effective treatment strategies. Deubiquitinating enzymes (DUBs), which regulate ubiquitin-dependent pathways, are potential targets for cancer therapy because of their structural advantages. Here we aimed to identify a potential target for HNSCC treatment among DUBs.
Methods: A screening process was conducted using RNA sequencing data and clinical information from HNSCC patients in the TCGA database. A panel of 88 DUBs was analyzed to identify those associated with poor prognosis. Subsequently, HNSCC cells were modified to overexpress specific DUBs, and their effects on cell proliferation and invasion were evaluated. In vivo experiments were performed to validate the findings.
Results: In HNSCC patients, USP10, USP14, OTUB1, and STAMBP among the screened DUBs were associated with a poor prognosis. Among them, OTUB1 showed the most aggressive characteristics in both in vitro and in vivo experiments. Additionally, OTUB1 regulated the stability and nuclear localization of YAP1, a substrate involved in cell proliferation and invasion. Notably, OTUB1 expression exhibited a positive correlation with the HNSCC-YAP score in HNSCC cells.
Conclusions: This study highlights the critical role of OTUB1 in HNSCC progression via modulating YAP1. Targeting the OTUB1-YAP1 axis holds promise as a potential therapeutic strategy for HNSCC treatment.
掲載誌名
Cancer Medicine
ISSN
20457634
出版者
John Wiley & Sons
12
24
開始ページ
22156
終了ページ
22169
発行日
2023-11-20
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shengjian Jinの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is an open access article under the terms of the Creative Commons Attribution License, (https://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3826号
学位記番号
甲口第501号
学位授与年月日
2024-03-22
学位名
博士(歯学)
学位授与機関
徳島大学
部局
医学系
歯学系