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ID 115668
著者
埴淵, 昌毅 Tokushima University|Shikoku Central Hospital of the Mutual Aid Association of Public School Teachers KAKEN研究者をさがす
Kanoh, Akira Taiho Pharmaceutical
Kuramoto, Takuya Taiho Pharmaceutical
Saito, Tatsuro Riken Genesis
香西, 博之 Tokushima University
近藤, 真代 Tokushima University
キーワード
non-small cell lung cancer
circulating free DNA
epidermal growth factor receptor mutation
epidermal growth factor receptor-tyrosine kinase inhibitor
資料タイプ
学術雑誌論文
抄録
The feasibility and required sensitivity of circulating free DNA (cfDNA)-based detection methods in second-line epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) treatment are not well elucidated. We examined T790M and other activating mutations of EGFR by cfDNA to assess the clinical usability. In 45 non-small cell lung cancer (NSCLC) patients harboring activating EGFR mutations, cfDNAs were prepared from the plasma samples. EGFR mutations in cfDNA were detected using highly sensitive methods and originally developed assays and these results were compared to tissue-based definitive diagnoses. The specificity of each cfDNA-based method ranged 96–100% whereas the sensitivity ranged 56–67%, indicating its low pseudo-positive rate. In EGFR-TKI failure cohort, 41–46% samples were positive for T790M by each cfDNA-based method, which was comparable to re-biopsy tissue-based T790M positive rates in literature. The concordance of the results for each EGFR mutation ranged from 83–95%. In eight patients, the results of the cfDNA-based assays and re-biopsy-derived tissue-based test were compared. The observed overall agreement ranged in 50–63% in T790M, and in 63–100% in activating EGFR mutations. In this study, we have newly developed three types of assay which have enough sensitivity to detect cfDNA. We also detected T790M in 44% of patients who failed prior EGFR-TKI treatment, indicating that cfDNA-based assay has clinical relevance for detecting acquired mutations of EGFR.
掲載誌名
Oncotarget
ISSN
19492553
出版者
Impact Journals
10
38
開始ページ
3654
終了ページ
3666
発行日
2019-06-04
権利情報
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0)(https://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
病院