Morimoto, Takaaki Kyoto University
Mineharu, Yohei Kyoto University
Ono, Koh Kyoto University
Nakatochi, Masahiro Nagoya University
Ichihara, Sahoko Mie University
Kabata, Risako Kyoto University
Cao, Yang Kyoto University|St. Marianna University School of Medicine
Zhao, Lanying Kyoto University
Kobayashi, Hatasu Kyoto University
Harada, Kouji H. Kyoto University
Takenaka, Katsunobu Takayama Red Cross Hospital
Funaki, Takeshi Kyoto University
Yokota, Mitsuhiro Aichi Gakuin University
Matsubara, Tatsuaki Aichi Gakuin University
Yamamoto, Ken Kurume University
Izawa, Hideo Fujita Health University
Kimura, Takeshi Kyoto University
Miyamoto, Susumu Kyoto University
Koizumi, Akio Kyoto University
The genetic architecture of coronary artery disease has not been fully elucidated, especially in Asian countries. Moyamoya disease is a progressive cerebrovascular disease that is reported to be complicated by coronary artery disease. Because most Japanese patients with moyamoya disease carry the p.R4810K variant of the ring finger 213 gene (RNF213), this may also be a risk factor for coronary artery disease; however, this possibility has never been tested.
Methods and results
We genotyped the RNF213 p.R4810K variant in 956 coronary artery disease patients and 716 controls and tested the association between p.R4810K and coronary artery disease. We also validated the association in an independent population of 311 coronary artery disease patients and 494 controls. In the replication study, the p.R4810K genotypes were imputed from genome-wide genotyping data based on the 1000 Genomes Project. We used multivariate logistic regression analyses to adjust for well-known risk factors such as dyslipidemia and smoking habits. In the primary study population, the frequency of the minor variant allele was significantly higher in patients with coronary artery disease than in controls (2.04% vs. 0.98%), with an odds ratio of 2.11 (p = 0.017). Under a dominant model, after adjustment for risk factors, the association remained significant, with an odds ratio of 2.90 (95% confidence interval: 1.37-6.61; p = 0.005). In the replication study, the association was significant after adjustment for age and sex (odds ratio = 4.99; 95% confidence interval: 1.16-21.53; p = 0.031), although it did not reach statistical significance when further adjusted for risk factors (odds ratio = 3.82; 95% confidence interval: 0.87-16.77; p = 0.076).
The RNF213 p.R4810K variant appears to be significantly associated with coronary artery disease in the Japanese population.
Copyright: © 2017 Morimoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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