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ID 115319
タイトル別表記
腸炎ビブリオにおけるトランスポゾン変異株を用いた長波長紫外線(UVA)感受性に関連する遺伝子の同定
Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticus
著者
安井, 実希 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Honjo, Airi Tokushima University
Bui, Thi Kim Ngan Tokushima University
キーワード
Vibrio parahaemolyticus
ultraviolet
light-emitting diodes
UV-LED
Oxidative stress
light-emitting diode
ultraviolet A
資料タイプ
学位論文
抄録
Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
掲載誌名
Applied Sciences
ISSN
20763417
出版者
MDPI
10
16
開始ページ
5549
発行日
2020-08-11
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Miki Maetani-Yasuiの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3462号
学位記番号
甲栄第281号
学位授与年月日
2020-09-24
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系
生物資源系
理工学系