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ID 119341
タイトル別表記
Dental Pulp Stem Cell Factors for Treating LF
著者
Hirata, Marina Nagoya University
Ishigami, Masatoshi Nagoya University
Matsushita, Yoshihiro Nagoya University
Ito, Takanori Nagoya University
Hattori, Hisashi Nagoya University
Hibi, Hideharu Nagoya University
Goto, Hidemi Nagoya University
Ueda, Minoru Nagoya University
キーワード
Liver fibrosis
Dental pulp stem cells
Conditioned media
Inflammation
Macrophages
資料タイプ
学術雑誌論文
抄録
Chronic liver injury from various causes often results in liver fibrosis (LF). Although the liver possesses endogenous tissue-repairing activities, these can be overcome by sustained inflammation and excessive fibrotic scar formation. Advanced LF leads to irreversible cirrhosis and subsequent liver failure and/or hepatic cancer. Here, using the mouse carbon tetrachloride (CCl4)-induced LF model, we showed that a single intravenous administration of stem cells derived from human exfoliated deciduous teeth (SHEDs) or of SHED-derived serum-free conditioned medium (SHED-CM) resulted in fibrotic scar resolution. SHED-CM suppressed the gene expression of proinflammatory mediators, such as TNF-α, IL-1β, and iNOS, and eliminated activated hepatic stellate cells by inducing their apoptosis, but protected parenchymal hepatocytes from undergoing apoptosis. In addition, SHED-CM induced tissue-repairing macrophages that expressed high levels of the profibrinolytic factor, matrix metalloproteinase 13. Furthermore, SHED-CM suppressed the CCl4-induced apoptosis of primary cultured hepatocytes. SHED-CM contained a high level of hepatocyte growth factor (HGF). Notably, HGF-depleted SHED-CM (dHGF-CM) did not suppress the proinflammatory response or resolve fibrotic scarring. Furthermore, SHED-CM, but not dHGF-CM, inhibited CCl4-induced hepatocyte apoptosis. These results suggest that HGF plays a central role in the SHED-CM-mediated resolution of LF. Taken together, our findings suggest that SHED-CM provides multifaceted therapeutic benefits for the treatment of LF.
掲載誌名
STEM CELLS Translational Medicine
ISSN
21576580
21576564
cat書誌ID
AA12597700
出版者
AlphaMed Press|Oxford University Press
5
10
開始ページ
1416
終了ページ
1424
発行日
2016-06-08
権利情報
STEM CELLS Translational Medicine® is a monthly, peer-reviewed, online only, open access journal.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系