Root canal treatment with high-frequency waves in rats
Matsui, Saori Osaka University
Yoneda, Naomichi Hyogo College of Medicine
Maezono, Hazuki Osaka University
Kuremoto, Katsutaka Osaka University
Ishimoto, Takuya Osaka University
Nakano, Takayoshi Osaka University
Ebisu, Shigeyuki Osaka University
Noiri, Yuichiro Niigata University
Hayashi, Mikako Osaka University
The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.
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pone_15_9_e0239660.pdf 1.92 MB