環境水試料中セレンの目視蛍光定量システムの構築

Selenium(IV) and Se(VI) were visually detected and determined based on fluorometric
derivatization used by 2,3-diaminonaphtalene (DAN) and β-cyclodextrin (β-CD) / sodium
dodecyl sulfate (SDS) as fluorometric enhancer. The visual determination of Se was achieved by
removing non-reacted DAN, which causes severe interferences and shows high background for
visual determination, with a cation exchange treatment. The cation exchange treatment provided
over 90% of its removal efficiency without loss of Se-DAN. In order to determine Se(VI), Se(VI)
was converted to Se(IV) based on the reduction of the treatment under 6 mol l-1 HCl at 110 °C.
Se(IV) and Se(VI) in a river water and a seawater sample could be quantitively recovered through
the Se-DAN derivatization and the cation exchange treatment. Under the optimal condition for
visual determination of Se, the fluorometric color was visually detected from 20 μg l-1 to 5000 μg l-1
of Se(IV) and from 100 μg l-1 to 5000 μg l-1 of Se(VI) and the difference of the color every 20-50 μg
l-1 was distinguished.