医学的応用に向けた細菌毒素に由来する機能性ナノバイオツールの作製

A novel functional protein tool derived from cholesterol-dependent cytolysin was developed and
its application for clinical field such as a drug delivery system was investigated. This tool is
composed of both of a functional-domain to connect the drug carrier and a targeting-domain to
recognize the target cell. The recombinant protein consisted of a lung tumor specific peptide,
domain 1-3 of intermedilysin with an introduced disulphide-bond to block the conformational
change and pore-formation, and domain 4 of Streptococcus mitis-derived human platelet
aggregation factor, was prepared using bacterial-expression system then investigated its function. It
was shown that the prepared protein tool could specifically deliver a model of drug-carrier vesicles
to human lung tumor cells in vitro. This result suggests that the novel functional protein tool
constructed in this study has the superior properties for clinical application.