ID 110681
著者
ナカムラ, ヤスオ Department of Dermatology, The University of Tokushima School of Medicine
イノウエ, マサヒロ Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima|Department of Parasitology, Kurume University School of Medicine
奥村, 裕司 Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima KAKEN研究者をさがす
シオタ, マユミ Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima
西川, 舞 Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima
荒瀬, 誠治 Department of Dermatology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
木戸, 博 Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
serine protease
testis
elongated spermatids
spermatogenesis
fertilization
資料タイプ
学術雑誌論文
抄録
Esp-1/testisin, a serine protease abundantly expressed in human and mouse testis, is presumed to play an important role in the process of spermatogenesis and fertilization. In this study, we cloned an esp-1/testisin cDNA from rats, and analyzed its expression and tissue distribution. The isolated cDNA consisted of 1099 nucleotides with a single open reading frame encoding 328 amino acids and an expected molecular mass of 36.6 kDa. The deduced amino acid sequence of rat Esp-1/Testisin had 89% and 62% identity with its murine and human counterparts, respectively, and appeared to be a trypsin-type serine protease with a hydrophobic region at the C-terminus. By quantitative real-time polymerase chain reaction analysis, rat esp-1/testisin mRNA was predominantly expressed in testis, as in human and mouse. However, its immunohistochemical distribution was predominantly in the elongated spermatids at steps 12 to 19, and not in the primary spermatocytes and round spermatids. This different distribution profile suggests that Esp-1/Testisin plays a role in species-specific proteolytic events during spermatogenesis and fertilization.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
50
1-2
開始ページ
78
終了ページ
86
並び順
78
発行日
2003
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所
医学系