アクセス数 : ?
ダウンロード数 : ?
ID 110741
著者
マツモト, ヨシヒト Department of Neurological Surgery, Kagawa University School of Medicine
ミヤケ, ケイスケ Department of Neurological Surgery, Kagawa University School of Medicine
クニシオ, カツゾウ Department of Neurological Surgery, Kagawa University School of Medicine
タミヤ, タカシ Department of Neurological Surgery, Kagawa University School of Medicine
ナガオ, セイゴ Department of Neurological Surgery, Kagawa University School of Medicine
キーワード
antisense
multidrug resistance
gene therapy
MRP
glioma
資料タイプ
学術雑誌論文
抄録
The tumor cells’ acquisition of resistance to multiple drugs due to overexpression of the multidrug resistance protein(MRP) 1gene is one of major obstacles in cancer chemotherapy. We have attempted to reverse the multidrug resistance (MDR) phenotype by treating etoposide resistant glioma cell lines (T98G-VP and Gli36-VP) with MRP1 antisense oligonucleotides. 20-mer phosphorothioate oligodeoxynucleotide (0.3μM), complementary to the coding region in the MRP cDNA sequence, could significantly inhibit the growth of multidrug resistant cell lines, T98G-VP and Gli36-VP, cultured in etoposide containing medium. No such effect was observed for the parental T98G and Gli36 cell lines. Further investigations by the reverse transcription-polymerase chain reaction and immunoblotting revealed that antisense oligomer could result in a reduction in the level of MRP1 mRNA, probably through hindering MRP1 gene transcription. This study demonstrates that the antisense oligonucleotides can increase the sensitivity of the tumor cells to the anticancer drug by decreasing the expression of the MRP gene. This strategy may be applicable to cure cancer patients with MRP mediated MDR phenotype.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
51
3-4
開始ページ
194
終了ページ
201
並び順
194
発行日
2004-08
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版