ID 110787
著者
シンドウ, トクヒサ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
マツモト, ヨシヒト Department of Neurological Surgery, Faculty of Medicine, Kagawa University
Wang, Qinghua Department of Neurological Surgery, Faculty of Medicine, Kagawa University
カワイ, ノブユキ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
タミヤ, タカシ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
ナガオ, セイゴ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
キーワード
embryonic stem cell
neural stem cell
transplantation
cholinergic
traumatic brain injury
資料タイプ
学術雑誌論文
抄録
We developed a novel protocol for generation and selective amplification of neural progenitor cells regionally specified to the rostral brain but not the spinal cord from mouse embryonic stem cells (ESCs). The neural progenitors could differentiate in vitro and in vivo into many cholinergic and a few GABAergic neurons but rarely into astrocytes. The transplanted neurospheres could survive in the hippocampus (CA3) of animals with mild traumatic brain injury (TBI). Twelve weeks after transplantation (a week after the behavioral test), we found significant cholinergic differentiation recognized as ChAT immunoreactivity in the eGFP+ transplanted cells. Moreover, the grafts contained a few GAD67+cells. However, we barely found GFAP+ astrocytes within the grafts. Furthermore, presynaptic formations of graft-derived neurons were recognized by immunohistochemistry of near the grafts aroundCA3. However, these findings were not observed in severe TBI group. So, we examined NGF, BDNF, and FGF-2 mRNA by RT-PCR in 12 mice including normal, mild TBI and severe TBI group. Increases in the neurotrophic factors’ mRNA were evident in the hippocampus on the ipsilateral side in the mild TBI group. Statistical analysis revealed significant differences between the mild and severe TBI groups. The data also revealed significant differences between the mild TBI and normal groups. The transplanted neurospheres could survive in the mild TBI animals, but not in the severe TBI group.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
53
1-2
開始ページ
42
終了ページ
51
並び順
42
発行日
2006-02
フルテキストファイル
言語
eng
著者版フラグ
出版社版