ID 110789
著者
Wang, Qinghua Department of Neurological Surgery, Faculty of Medicine, Kagawa University
マツモト, ヨシヒト Department of Neurological Surgery, Faculty of Medicine, Kagawa University
シンドウ, トクヒサ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
ミヤケ, ケイスケ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
シンドウ, アツシ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
カワニシ, マサヒコ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
カワイ, ノブユキ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
タミヤ, タカシ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
ナガオ, セイゴ Department of Neurological Surgery, Faculty of Medicine, Kagawa University
キーワード
neurosphere
embryonic stem cell
nucleus basalis of Meynert
dementia
mouse
資料タイプ
学術雑誌論文
抄録
Objective. The goal of this study was to elucidate the effect of neurospheres (NS) on dementia in the mouse model of nucleus basalis of Meynert (NBM) lesion. Methods. Mouse embryonic stem cell (ES) derived neurospheres were transplanted into the frontal association cortex and barrel field of S1 cortex of C57BL/6mice 4weeks after including a lesion of NBM by ibotenic acid, while other healthy mice that received ES cells served as control. Behavioral tests by 8-armradial maze were conducted 8 weeks after transplantation, and double staining of choline acetyltransferase (ChAT), serotonin, amyloid-βprotein (AP) and green fluorescent protein(GFP)12 weeks after transplantation.We found that the neurospheres transplanted into the mouse cortex survived and produced many ChAT-positive neurons and a few serotoninpositive neurons in and around the grafts. The working memory error decreased significantly in the mice grafted with neurospheres. In contrast, the ES cells developed into teratomas in all of the control mice and expressed no neurons, and the working memory deteriorated remarkably. Conclusions. Transplantation of neurospheres, but not ES cells, into the prefrontal and parietal cortices, dramatically alleviated the cholinergic deficits and recent memory disruption in the NBM lesioned mice.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
53
1-2
開始ページ
61
終了ページ
69
並び順
61
発行日
2006-02
フルテキストファイル
言語
eng
著者版フラグ
出版社版