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ID 111308
著者
Karabasil, Mileva Ratko The University of Tokushima
Azlina, Ahmad The University of Tokushima
Purwanti, Nunuk The University of Tokushima
Purevjav, Javkhlan The University of Tokushima
姚, 陳娟 The University of Tokushima
キーワード
aquaporin-5
PKA-consensus sequence
trafficking
資料タイプ
学術雑誌論文
抄録
Three constructs having mutated PKA-target motif at 152SRRTS of AQP5, an exocrine type water channel, were prepared and fused to C-terminus of green fluorescence protein cDNA to examine the effects of blocking of phosphorylation at 152SRRTS (a consensus PKA-target motif of AQP5) on translocation or trafficking of the chimeric proteins expressed in the Madin-Darby canine kidney-II (MDCK-II) cells. H-89 treatment increased translocation of wild-type GFP-AQP5 to the apical membrane. All 3 mutant molecules translocated 1.5 to 2 times more than the control wild-type GFP-AQP5. Colchicine but not cytochalasin B inhibited the translocation of wild-type GFP-AQP5. Present results suggest dephosphorylation of this consensus sequence increase GFP-AQP5 translocation, and that microtubules but not microfilaments are involved in this event.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
1-2
開始ページ
55
終了ページ
63
並び順
55
発行日
2009-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系
生物資源系