ID 111333
著者
Inenaga, Kiyotoshi Kyushu Dental College
Yamada, Naoyuki Ajinomoto Co.
Yuji, Reiko Ajinomoto Co.
Kawai, Misako Ajinomoto Co.
Uneyama, Hisayuki Ajinomoto Co.
Ono, Kentaro Kyushu Dental College
Suzuki, Ei-ichiro Ajinomoto Co.
Torii, Kunio Ajinomoto Co.
キーワード
proteome
saliva
malnutrition
biomarker
資料タイプ
学術雑誌論文
抄録
Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
224
終了ページ
227
並び順
224
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版