Inenaga, Kiyotoshi Kyushu Dental College
Yamada, Naoyuki Ajinomoto Co.
Yuji, Reiko Ajinomoto Co.
Kawai, Misako Ajinomoto Co.
Uneyama, Hisayuki Ajinomoto Co.
Ono, Kentaro Kyushu Dental College
Suzuki, Ei-ichiro Ajinomoto Co.
Torii, Kunio Ajinomoto Co.
Proteome analysis is a popular method to discover biomarkers for the prevention and diagnosis of diseases. Since saliva is a non-invasively available body fluid, gathering of saliva causes minimal harm to patients. Therefore, detection of proteins for the prevention and diagnosis from the saliva sample may be the preferred method, especially for children and elderly people. However, the abundance of salivary proteins and contaminant proteins from food and mouth bacteria obscure identification of proteins present in the saliva at low concentrations. To address this problem, we developed a shotgun proteomic method using two-dimensional nano-flow LC tandem mass spectrometry. We report here that our method is able to detect proteins quantitatively even in small sample volumes of saliva.
The Journal of Medical Investigation
Faculty of Medicine Tokushima University
jmi_56_suppl_224.pdf 147 KB