ID 111459
著者
Ichikawa, Hideki Tokyo Dental College
Shibukawa, Yoshiyuki Tokyo Dental College
Sahara, Yoshinori Iwate Medical University
Tsumura, Maki Tokyo Dental College|Toho University
Qi, Bing Nihon University
Satoh, Keitaro Dokkyo Medical University
Narita, Takanori Nihon University
Hashimoto, Sadamitsu Tokyo Dental College
Momose, Yasunori Toho University
Tazaki, Masakazu Tokyo Dental College
Shimono, Masaki Tokyo Dental College
Sugiya, Hiroshi Nihon University
キーワード
AQP6
parotid acinar cells
ion transport
資料タイプ
学術雑誌論文
抄録
Salivary gland acinar cells secrete large amounts of water and electrolytes, where aquaporins (AQPs) are thought to be involved in the secretion. In the present study, we investigated expression/localization of AQP6, and the anion transporting properties of AQP6 in mouse parotid acinar cells. RT-PCR, western blotting and immunohistochemical analyses revealed expression of AQP6 in acinar cells, localized in apical membrane. Voltage ramp from -100 mV to +100 mV at a holding potential of -60 mV elicited outwardlyrectifying currents, in the presence of extracellular Cl- channel blockers and intracellular solution with 150 mM Cs+. These outward currents were increased when extracellular Cl- was replaced by Br-, NO3-, I-, or SCN-, accompanying a negative shift of reversal potentials. The outward current was enhanced by extracellular Hg2+. These results were consistent with the biophysical properties of transfected AQP6 oocytes or HEK cells, which indicate that the AQP6 channel is functionally expressed in parotid acinar cells, and suggest that AQP6 contributes to secretion of anions in parotid acinar cells.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
347
終了ページ
349
並び順
347
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版