ID 111478
著者
Bovell, Douglas L Glasgow Caledonian University
Kofler, Barbara Paracelsus Medical University
Lang, Roland Paracelsus Medical University
キーワード
proteinase activated receptors
eccrine sweat glands
ussing chamber
immunofluorescence
資料タイプ
学術雑誌論文
抄録
Serine proteases can induce cell signaling by stimulating G-protein-coupled receptors, called proteinase-activated receptors (PAR’s) on a variety of epithelial cells. While PAR-2, one such receptor, activates cell signaling in a secretory cell line derived from human sweat glands, there was no information on their presence and effects on intact sweat glands. PAR-2 presence and activation of eccrine sweat glands isolated from human skin samples was investigated using Western blot analysis, immunohistochemistry, electron microscopy (EM) and Ca2+ imaging. Anti-human PAR-2 antibody demonstrated the presence of these receptors in eccrine sweat glands. EM showed that PAR-2 activation resulted in degranulation of secretory cells. Ca2+ imaging using PAR-2 activators demonstrated a two phase increase in [Ca2+]i which was dependent on extracellular Ca2+ for the second phase, and that the response could be blocked by prior incubation with xestospongin, the IP3 receptor blocker. The results demonstrated that PAR-2 receptors are present in human sweat gland secretory cells and that these receptors are functionally active and can induce changes associated with secretory events in eccrine glands.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
371
終了ページ
374
並び順
371
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版