ID 111480
著者
Iida, Taichi Kyushu Dental College
Ono, Kentaro Kyushu Dental College
Inagaki, Tomohiro Kyushu Dental College
Hosokawa, Ryuji Kyushu Dental College
Inenaga, Kiyotoshi Kyushu Dental College
キーワード
nicotine
nerve terminal
parotid cells
資料タイプ
その他
抄録
Nicotine has been reported to increase the intracellular Ca2+ concentration ([Ca2+]i) in sublingual acini due to neurotransmitter release from nerve terminals associated with the cell preparation (1). However, it is unclear whether or not the same reaction exists in parotid cells. Therefore, we investigated effect of nicotine on Ca2+ mobilization in digested parotid acini from rats. After removing the parotid gland from Wistar rats, the tissues were minced and digested with collagenase. Then, the intracellular Ca2+ indicator fura-2 was added to the preparation, and the change in [Ca2+]i was monitored using fluorescent microscope. In many but not all parotid acini, K+ stimulation induced transient increases in [Ca2+]i. The K+-induced Ca2+ response in parotid acini was completely blocked by Cd2+-containing solution. These results suggest that the parotid cell preparation has nerve terminals. In all high-K+-sensitive parotid acini, over 3μM of nicotine increased [Ca2+]i, and the response was blocked by a Cd2+-containing solution and nicotinic receptor antagonists. All high-K+-insensitive acinar cells were resistant to the effect of nicotine on Ca2+ mobilization. These results suggest that nicotine induces increases in [Ca2+]i in parotid acini due to neurotransmitter release from associated nerve terminals.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
Supplement
開始ページ
376
終了ページ
376
並び順
376
発行日
2009-12
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版