ID 111181
タイトル別表記
機能的マーカーとしての活性化NK細胞表面CD56発現のフローサイトメトリー評価
Evaluation of Surface CD56 Expression
著者
大星, 航 徳島大学大学院保健科学教育部(保健学専攻)
Tada, Tomoki Tokushima University
Watanabe, Toru Kagawa Prefectural University of Health Sciences
Yukimasa, Nobuyasu Kagawa Prefectural University of Health Sciences
Ueno, Ichiro Kagawa Prefectural University of Health Sciences
キーワード
natural killer (NK) cells
CD56
cytotoxicity
IFN-γmRNA
flow cytometry
資料タイプ
学位論文
抄録
Surface CD56 is the most important cell marker for defining NK cells. However, the relationship between the expression of surface CD56 and NK cell activity has not yet been elucidated in detail. Thirteen healthy volunteers were enrolled in the present study. Peripheral blood mononuclear cells (PBMCs) were stimulated with rIL-2 or rIL-12 (1, 10, 100 U/mL) for 18 h at 37 ℃. After incubation, surface CD56 expression on NK cells was evaluated using a flow cytometric analysis. A colorimetric-based lactate dehydrogenase (LDH) assay was used for experiments on cytotoxicity. IFN-γmRNA gene expression was quantified by real-time PCR. The expression level of surface CD56 on NK cells, cytotoxicity, and IFN-γmRNA gene expression were significantly increased by the rIL-2 and rIL-12 stimulations. In addition, a positive correlation was found between surface CD56 expression and cytotoxic activity or IFN-γmRNA gene expression. We revealed that the quantification of surface CD56 expression was applicable to the evaluation of cytotoxicity and IFN-γproduction in activated NK cells. These results suggest that the measurement of surface CD56 expression represent an easy and rapidly reproducible technique to evaluate the activated state of NK cells and monitor NK cell activity in immunotherapy.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
63
3-4
開始ページ
199
終了ページ
203
並び順
199
発行日
2016-08
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201611141022.pdf
審査要旨 : LID201611141023.pdf
論文本文 : LID201611141024.pdf
本論文は,著者Wataru Oboshiの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2975号
学位記番号
甲保第24号
学位授与年月日
2016-09-15
学位名
博士(保健学)
学位授与機関
徳島大学
部局
医学系