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ID 109793
タイトルヨミ
バイヨウ シズイ サイボウ ニオケル Streptococcus mutans オヨビ エンショウセイ サイトカイン ニヨル CCL20 ノ ハツゲン
タイトル別表記
CCL20 Production is Induced in Human Dental Pulp upon Stimulation by Streptococcus Mutans and Proinflammatory Cytokines
著者
高橋, 加奈子 徳島大学大学院ヘルスバイオサイエンス研究部発達予防医歯学部門健康長寿歯科学講座歯科保存学分野
キーワード
CCL20
内皮細胞
歯髄線維芽細胞
歯髄炎
資料タイプ
学術雑誌論文
抄録
Introduction: Pulpitis is characterized as a marked infiltration of inflammatory cells in response to an invasion of caries-related bacteria. It is well known that chemokines regulate the trafficking of lymphocytes, and CC chemokine ligand 20 (CCL20) has been recently shown to play a crucial role in the recruitment of memory T cells and immature dendritic cells into inflammatory lesions. We previously reported that CCL20 was mainly expressed in microvasucular endothelial cells and macrophages accumulated in inflamed pulp tissues and its specific receptor; CCR6, was expressed on infiltrated lymphocytes. However, the mechanism of CCL20 expression remains unclear.
Methods and Results: In this study, we investigated the expression of CCL20 in monocytes/macrophages, endothelial cells and pulpal fibroblasts after stimulation with Streptococcus mutans, a representative of caries-related bacteria, or pro-inflammatory cytokines. CCL20 mRNA was detected in inflamed pulp, but not in clinically normal pulp by reverse transcription-polymerase chain reaction. S. mutans induced human monocytic cells (THP-1), macrophage-like THP-1 differentiated with phorbol myristate acetate, and human umbilical vein endothelial cells (HUVEC) to produce an increased level of CCL20 as demonstrated by enzyme-linked immunosorbent assay. Lipoteichoic acid from S. mutans also elicited CCL20 production by HUVEC. Moreover, CCL20 production from cultured pulpal fibroblasts was increased by the stimulation with inetrleukin-1β and tumor necrosis factor-α.
Conclusion: Our results indicate that CCL20 expression is induced by stimulation with caries-related bacteria invaded deeply into dentinal tubules and pro-inflammatory cytokines in inflamed pulpal lesions and may be involved in the progression of pulpitis via accumulation of inflammatory cells.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
出版者
四国歯学会
22
2
開始ページ
157
終了ページ
165
並び順
157
発行日
2010-01-31
フルテキストファイル
言語
jpn
著者版フラグ
出版社版