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ID 109815
タイトルヨミ
タンパクシツ リンサンカ ト ダツリンサンカ ニヨル コツケイセイ ト コツキュウシュウ
タイトル別表記
Protein Kinases and Protein Phosphatases in Bone Formation and Bone Resorption
著者
羽地, 達次 徳島大学大学院ヘルスバイオサイエンス研究部口腔組織学分野 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
PP1δ
PKR
C23
骨芽細胞
破骨細胞
資料タイプ
学術雑誌論文
抄録
Serine and threonine protein phosphatases (PP) are divided into four categories, PP1, PP2A, PP2B, and PP2C. cDNA cloning revealed the existence of at least four isoforms of PP1 catalytic subunit in rat, termed PP1α, PP1γ1, PP1γ2, and PP1δ. Aanti-PP1δ antibody recognized a protein present in the nucleolar regions in human and mouse osteoblasts. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. C23 (nucleolin) is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of C23 in human osteoblasts was similar to that of the PP1δ. PP1δ and C23 were specifically stained as dots in the nucleus. The dual fluorescence images revealed that PP1δ and C23 were localized in the same regions in the nucleolus. These results indicate that PP1δ associate with C23 directly in the nucleolus and suggest that C23 is one of the candidates of PP1δ substrates.
Double-stranded RNA-dependent protein kinase (PKR) is a serine/threonine protein kinase expressed in mammalian cells. PKR is activated by double-stranded RNA (dsRNA), interferon, cytokines, stress signals, and viral infections. Dominant-negative mutant PKR cDNA, in which the amino acid lysine at 296 was replaced with arginine and which does not have catalytic activity, was constructed. The mutant cDNA was transfected into mouse osteoblastic MC3T3-E1 cells and preosteoclastic mouse macrophage RAW264.7 cells. Using these cells, our group demonstrated that PKR plays important roles in the differentiation and calcification of osteoblasts by modulating STAT1α and/or Runx2 expression. In this review, I also described that PKR is required for the osteoclast formation of preosteoclastic cell by fusing these cells to form multi-nucleated giant cells.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
出版者
四国歯学会
24
2
開始ページ
45
終了ページ
50
並び順
45
発行日
2012-01-31
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
歯学系