アクセス数 : ?
ダウンロード数 : ?
ID 112395
タイトル別表記
等張または低張条件下のラット耳下腺腺房細胞におけるα1-アドレナリン受容体誘導性のAQP5細胞内移動機序
著者
ブラジエル, アネタ マグダレナ 徳島大学大学院口腔科学教育部(口腔科学専攻)
Wang, Di Tokushima University|Fourth Military Medical University
Pieczonka, Tomasz D. Tokushima University
キーワード
aquaporin-5
α1A-adrenoceptor
α1B-adrenoceptor
α1D-adrenoceptor
calcium
protein G kinase
hypotonicity
資料タイプ
学位論文
抄録
Defective cellular trafficking of aquaporin-5 (AQP5) to the apical plasma membrane (APM) in salivary glands is associated with the loss of salivary fluid secretion. To examine mechanisms of α1-adrenoceptor (AR)-induced trafficking of AQP5, immunoconfocal microscopy and Western blot analysis were used to analyze AQP5 localization in parotid tissues stimulated with phenylephrine under different osmolality. Phenylephrine-induced trafficking of AQP5 to the APM and lateral plasma membrane (LPM) was mediated via the α1A-AR subtype, but not the α1B- and α1D-AR subtypes. Phenylephrine-induced trafficking of AQP5 was inhibited by ODQ and KT5823, inhibitors of nitric oxide (NO)-stimulated guanylcyclase (GC) and protein kinase (PK) G, respectively, indicating the involvement of the NO/ soluble (c) GC/PKG signaling pathway. Under isotonic conditions, phenylephrine-induced trafficking was inhibited by La3+, implying the participation of store-operated Ca2+ channel. Under hypotonic conditions, phenylephrine-induced trafficking of AQP5 to the APM was higher than that under isotonic conditions. Under non-stimulated conditions, hypotonicity-induced trafficking of AQP5 to the APM was inhibited by ruthenium red and La3+, suggesting the involvement of extracellular Ca2+ entry. Thus, α1A-AR activation induced the trafficking of AQP5 to the APM and LPM via the Ca2+/ cyclic guanosine monophosphate (cGMP)/PKG signaling pathway, which is associated with store-operated Ca2+ entry.
掲載誌名
International Journal of Molecular Sciences
ISSN
14220067
16616596
cat書誌ID
AA12038549
出版者
MDPI
17
7
開始ページ
1022
発行日
2016-06-28
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704251013.pdf
審査要旨:LID201704251014.pdf
論文本文:LID201704251015.pdf
本論文は,著者Aneta M. Bragielの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3071号
学位記番号
甲口第414号
学位授与年月日
2017-03-23
学位名
博士(学術)
学位授与機関
徳島大学
部局
技術支援部
歯学系