直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 119194
タイトル別表記
Promoter elements for restricted expression of Hes7
著者
林, 真一 Nara Institute of Science and Technology|Tokushima University KAKEN研究者をさがす
Nakahata, Yasukazu Nara Institute of Science and Technology
Kohno, Kenji Nara Institute of Science and Technology
Matsui, Takaaki Nara Institute of Science and Technology
Bessho, Yasumasa Nara Institute of Science and Technology
資料タイプ
学術雑誌論文
抄録
Somites are a pair of epithelial spheres beside a neural tube and are formed with an accurate periodicity during embryogenesis in vertebrates. It has been known that Hes7 is one of the core clock genes for somitogenesis, and its expression domain is restricted in the presomitic mesoderm (PSM). However, the molecular mechanism of how Hes7 transcription is regulated is not clear. Here, using transgenic mice and luciferase-based reporter assays and in vitro binding assays, we unravel the mechanism by which Hes7 is expressed exclusively in the PSM. We identified a Hes7 essential region residing-1.5 to-1.1 kb from the transcription start site of mouse Hes7, and this region was indispensable for PSM-specific Hes7 expression. We also present detailed analyses of cis-regulatory elements within the Hes7 essential region that directs Hes7 expression in the PSM. Hes7 expression in the PSM was up-regulated through the E-box, T-box, and RBPj-binding element in the Hes7 essential region, presumably through synergistic signaling involving mesogenin1, T-box6 (Tbx6), and Notch. Furthermore, we demonstrate that Tbx18, Ripply2, and Hes7 repress the activation of the Hes7 essential region by the aforementioned transcription factors. Our findings reveal that a unified transcriptional regulatory network involving a Hes7 essential region confers robust PSM-specific Hes7 gene expression.
掲載誌名
Journal of Biological Chemistry
ISSN
00219258
1083351X
cat書誌ID
AA1202441X
出版者
American Society for Biochemistry and Molecular Biology|Elsevier
293
31
開始ページ
12167
終了ページ
12176
発行日
2018-06-12
権利情報
This is an Open Access article under the CC BY license(https://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所