直近一年間の累計
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ダウンロード数 : ?
ID 111507
著者
Piroozmand, Ahmad The University of Tokushima|Kashan University of Medical Sciences
Yamamoto, Yoshihiko Osaka National Hospital
Khamsri, Boonruang The University of Tokushima
Fujita, Mikako The University of Tokushima
内山, 恒夫 The University of Tokushima KAKEN研究者をさがす
キーワード
HIV-1
Vif
APOBEC3G
293T
資料タイプ
学術雑誌論文
抄録
We have established a number of 293T cell lines that express a human anti HIV-1 factor APOBEC3G. Out of seven cell clones examined, four were readily demonstrated to express APOBEC3G by immunoblotting analysis. In particular, two clones (A3G-C1 and -C4) were found to produce a much higher level of functional APOBEC3G relative to that by pooled cell clones. The transfection efficiency of all these cell clones were similar to that of the parental cells, producing a comparable level of virions upon transfection of wild type and vif -minus proviral DNA clones. Furthermore, the expression level of APOBEC3G in the best cell line (A3G-C1) was far much higher than those of an APOBEC3G-positive lymphocyte cell line and peripheral blood mononuclear cells. We finally monitored the incorporation of APOBEC3G into virions produced in A3G-C1. APOBEC3G was easily detected in progeny viral particles upon transfection of vif -minus proviral clone but not of wild type. These results indicated that our new A3G-C1 cell line is eminently useful for various studies on the interaction of human APOBEC3G and HIV-1 Vif.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
54
1-2
開始ページ
154
終了ページ
158
並び順
154
発行日
2007-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系