ID | 111314 |
Author |
Tsuchigauchi, Takeshi
The University of Tokushima
Ohnishi, Takamasa
The University of Tokushima
Ogawa, Hirohisa
The University of Tokushima
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Bando, Yoshimi
The University of Tokushima
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Uehara, Hisanori
The University of Tokushima
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Takizawa, Tamotsu
The University of Tokushima
Kaneda, Shinya
The University of Tokushima
Nakai, Tokiko
The University of Tokushima
Shiota, Hiroshi
The University of Tokushima
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Izumi, Keisuke
The University of Tokushima
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Keywords | Long-Evans Cinnamon rat
Long-Evans Agouti rat
N-ethyl-N-nitrosourea
trigeminal schwannoma
metallothionein
O6-methylduanine-DNA methyltransferase
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Content Type |
Journal Article
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Description | The Long-Evans Cinnamon (LEC) rat, an animal model of Wilson’s disease, is resistant to a variety of chemical carcinogenesis except liver and colon. In the present study, N-ethyl-N-nitrosourea (ENU)-induced transplacental carcinogenesis was examined in male and female LEC, Long-Evans Agouti (LEA), a sibling line of the LEC rat, and F344 rats (n=21). ENU was administered to pregnant rats as a single s.c. injection at a dose of 60 mg/kg body weight on the 17th day after conception. Cerebral/spinal gliomas and trigeminal/spinal nerve schwannomas developed in both LEA and F344 rats at 30 weeks of age, but no nervous system tumors developed in LEC rats, the difference being statistically significant. Lung adenomas also developed in LEA and F344 rats, but not in LEC rats. Semiquantitative RT-PCR demonstrated that metallothionein (MT)1a, MT2 and O6-methylguanine-DNA methyltransferase (MGMT) mRNA levels in the liver of LEC rats were higher than those in F344 and LEA rats. In addition, Western blot analysis showed that MT (MT1 plus MT2) in the liver of LEC rats was also higher than that in other strains. Present results suggest that high levels of MT and/or MGMT contribute to the resistance to nitrosamine-induced carcinogenesis in LEC rats.
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Journal Title |
The Journal of Medical Investigation
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ISSN | 13496867
13431420
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NCID | AA11166929
AA12022913
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Publisher | Faculty of Medicine Tokushima University
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Volume | 56
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Issue | 3-4
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Start Page | 93
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End Page | 98
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Sort Key | 93
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Published Date | 2009-08
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Publisher
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departments |
Medical Sciences
University Hospital
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