ID 111314
著者
Tsuchigauchi, Takeshi The University of Tokushima
髙橋, 徹行 The University of Tokushima KAKEN研究者をさがす
Ohnishi, Takamasa The University of Tokushima
Takizawa, Tamotsu The University of Tokushima
Kaneda, Shinya The University of Tokushima
Nakai, Tokiko The University of Tokushima
キーワード
Long-Evans Cinnamon rat
Long-Evans Agouti rat
N-ethyl-N-nitrosourea
trigeminal schwannoma
metallothionein
O6-methylduanine-DNA methyltransferase
資料タイプ
学術雑誌論文
抄録
The Long-Evans Cinnamon (LEC) rat, an animal model of Wilson’s disease, is resistant to a variety of chemical carcinogenesis except liver and colon. In the present study, N-ethyl-N-nitrosourea (ENU)-induced transplacental carcinogenesis was examined in male and female LEC, Long-Evans Agouti (LEA), a sibling line of the LEC rat, and F344 rats (n=21). ENU was administered to pregnant rats as a single s.c. injection at a dose of 60 mg/kg body weight on the 17th day after conception. Cerebral/spinal gliomas and trigeminal/spinal nerve schwannomas developed in both LEA and F344 rats at 30 weeks of age, but no nervous system tumors developed in LEC rats, the difference being statistically significant. Lung adenomas also developed in LEA and F344 rats, but not in LEC rats. Semiquantitative RT-PCR demonstrated that metallothionein (MT)1a, MT2 and O6-methylguanine-DNA methyltransferase (MGMT) mRNA levels in the liver of LEC rats were higher than those in F344 and LEA rats. In addition, Western blot analysis showed that MT (MT1 plus MT2) in the liver of LEC rats was also higher than that in other strains. Present results suggest that high levels of MT and/or MGMT contribute to the resistance to nitrosamine-induced carcinogenesis in LEC rats.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
56
3-4
開始ページ
93
終了ページ
98
並び順
93
発行日
2009-08
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
病院