ID 111314
Author
Tsuchigauchi, Takeshi The University of Tokushima
Takahashi, Tetsuyuki The University of Tokushima KAKEN Search Researchers
Ohnishi, Takamasa The University of Tokushima
Takizawa, Tamotsu The University of Tokushima
Kaneda, Shinya The University of Tokushima
Nakai, Tokiko The University of Tokushima
Keywords
Long-Evans Cinnamon rat
Long-Evans Agouti rat
N-ethyl-N-nitrosourea
trigeminal schwannoma
metallothionein
O6-methylduanine-DNA methyltransferase
Content Type
Journal Article
Description
The Long-Evans Cinnamon (LEC) rat, an animal model of Wilson’s disease, is resistant to a variety of chemical carcinogenesis except liver and colon. In the present study, N-ethyl-N-nitrosourea (ENU)-induced transplacental carcinogenesis was examined in male and female LEC, Long-Evans Agouti (LEA), a sibling line of the LEC rat, and F344 rats (n=21). ENU was administered to pregnant rats as a single s.c. injection at a dose of 60 mg/kg body weight on the 17th day after conception. Cerebral/spinal gliomas and trigeminal/spinal nerve schwannomas developed in both LEA and F344 rats at 30 weeks of age, but no nervous system tumors developed in LEC rats, the difference being statistically significant. Lung adenomas also developed in LEA and F344 rats, but not in LEC rats. Semiquantitative RT-PCR demonstrated that metallothionein (MT)1a, MT2 and O6-methylguanine-DNA methyltransferase (MGMT) mRNA levels in the liver of LEC rats were higher than those in F344 and LEA rats. In addition, Western blot analysis showed that MT (MT1 plus MT2) in the liver of LEC rats was also higher than that in other strains. Present results suggest that high levels of MT and/or MGMT contribute to the resistance to nitrosamine-induced carcinogenesis in LEC rats.
Journal Title
The Journal of Medical Investigation
ISSN
13496867
13431420
NCID
AA11166929
AA12022913
Publisher
Faculty of Medicine Tokushima University
Volume
56
Issue
3-4
Start Page
93
End Page
98
Sort Key
93
Published Date
2009-08
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Medical Sciences
University Hospital