Analysis of different complexes of type IIa sodium-dependent phosphate transporter in rat renal cortex using blue-native polyacrylamide gel electrophoresis

Tanimura, Ayako; Yamada, Fumiyo; Saito, Akihito; Ito, Mikiko; Kimura, Toru; Anzai, Naohiko; Horie, Daisuke; Yamamoto, Hironori; Miyamoto, Ken-ichi; Taketani, Yutaka; Takeda, Eiji

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ID	82750
Author	Tanimura, Ayako Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers Yamada, Fumiyo Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Saito, Akihito Department of Applied Molecular Medicine, Niigata University Graduate School of Medicine and Dental Sciences Ito, Mikiko Department of Food Science and Nutrition, School of Human Science and Environment, University of Hyogo Kimura, Toru Department of Pharmacology,School of Medicine, Kyorin University Anzai, Naohiko Department of Pharmacology,School of Medicine, Kyorin University Horie, Daisuke Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Yamamoto, Hironori Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers Miyamoto, Ken-ichi Department of Molecular Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers Taketani, Yutaka Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers Takeda, Eiji Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords	native PAGE macromolecular complex NHERF1 PDZK1 megalin
Content Type	Journal Article
Description	Type IIa sodium-dependent phosphate transporter (NaPi-IIa) can be localized in the apical plasma membrane of renal proximal tubule to carry out a rate-limiting step of phosphate reabsorption. For the apical localization, NaPi-IIa is required to form a macromolecular complex with some adaptor proteins such as Na+/H+ exchanger regulatory factor 1 (NHERF-1) and ezrin. However, the detail of macromolecular complex containing NaPi-IIa in the apical membrane of the renal proximal tubular cells has not been clarified. In this study, we identified at least four different complexes (220, 480, 920, 1,100 kDa) containing NaPi-IIa by using blue-native polyacrylamide gel electrophoresis. Interestingly, LC-MS/MS analysis and immunoprecipitation analysis reveal that megalin is a component of larger complexs (920 and 1,100 kDa). In addition, NaPi-IIa can be heterogeneously co-localized with ezrin and megalin on the apical membrane of renal proximal tubuler cells by fluorescence microscopy analysis. These results suggest that NaPi-IIa can form some different complexes on the apical plasma membrane of renal proximal tubular cells.
Journal Title	The journal of medical investigation : JMI
ISSN	13431420
NCID	AA11166929
Volume	58
Issue	1-2
Start Page	140
End Page	147
Sort Key	140
Published Date	2011-02
Remark	The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID	241403
FullText File	LID201112092006.pdf 330 KB
language	eng
departments	Oral Sciences Medical Sciences