ID 82750
著者
谷村, 綾子 Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
ヤマダ, フミヨ Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
サイトウ, アキヒト Department of Applied Molecular Medicine, Niigata University Graduate School of Medicine and Dental Sciences
伊藤, 美紀子 Department of Food Science and Nutrition, School of Human Science and Environment, University of Hyogo
キムラ, トオル Department of Pharmacology,School of Medicine, Kyorin University
アンザイ, ナオヒコ Department of Pharmacology,School of Medicine, Kyorin University
ホリエ, ダイスケ Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
山本, 浩範 Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN研究者をさがす
宮本, 賢一 Department of Molecular Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
竹谷, 豊 Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
武田, 英二 Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
native PAGE
macromolecular complex
NHERF1
PDZK1
megalin
資料タイプ
学術雑誌論文
抄録
Type IIa sodium-dependent phosphate transporter (NaPi-IIa) can be localized
in the apical plasma membrane of renal proximal tubule to carry out a rate-limiting step
of phosphate reabsorption. For the apical localization, NaPi-IIa is required to form a
macromolecular complex with some adaptor proteins such as Na+/H+ exchanger regulatory
factor 1 (NHERF-1) and ezrin. However, the detail of macromolecular complex containing
NaPi-IIa in the apical membrane of the renal proximal tubular cells has not been
clarified. In this study, we identified at least four different complexes (220, 480, 920, 1,100
kDa) containing NaPi-IIa by using blue-native polyacrylamide gel electrophoresis. Interestingly,
LC-MS/MS analysis and immunoprecipitation analysis reveal that megalin
is a component of larger complexs (920 and 1,100 kDa). In addition, NaPi-IIa can be heterogeneously
co-localized with ezrin and megalin on the apical membrane of renal proximal
tubuler cells by fluorescence microscopy analysis. These results suggest that NaPi-IIa
can form some different complexes on the apical plasma membrane of renal proximal tubular
cells.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
58
1-2
開始ページ
140
終了ページ
147
並び順
140
発行日
2011-02
備考
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
241403
フルテキストファイル
言語
eng
部局
歯学系
医学系