ID 82750
Author
Tanimura, Ayako Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Yamada, Fumiyo Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
Saito, Akihito Department of Applied Molecular Medicine, Niigata University Graduate School of Medicine and Dental Sciences
Ito, Mikiko Department of Food Science and Nutrition, School of Human Science and Environment, University of Hyogo
Kimura, Toru Department of Pharmacology,School of Medicine, Kyorin University
Anzai, Naohiko Department of Pharmacology,School of Medicine, Kyorin University
Horie, Daisuke Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
Yamamoto, Hironori Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School KAKEN Search Researchers
Miyamoto, Ken-ichi Department of Molecular Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Taketani, Yutaka Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Takeda, Eiji Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
native PAGE
macromolecular complex
NHERF1
PDZK1
megalin
Content Type
Journal Article
Description
Type IIa sodium-dependent phosphate transporter (NaPi-IIa) can be localized
in the apical plasma membrane of renal proximal tubule to carry out a rate-limiting step
of phosphate reabsorption. For the apical localization, NaPi-IIa is required to form a
macromolecular complex with some adaptor proteins such as Na+/H+ exchanger regulatory
factor 1 (NHERF-1) and ezrin. However, the detail of macromolecular complex containing
NaPi-IIa in the apical membrane of the renal proximal tubular cells has not been
clarified. In this study, we identified at least four different complexes (220, 480, 920, 1,100
kDa) containing NaPi-IIa by using blue-native polyacrylamide gel electrophoresis. Interestingly,
LC-MS/MS analysis and immunoprecipitation analysis reveal that megalin
is a component of larger complexs (920 and 1,100 kDa). In addition, NaPi-IIa can be heterogeneously
co-localized with ezrin and megalin on the apical membrane of renal proximal
tubuler cells by fluorescence microscopy analysis. These results suggest that NaPi-IIa
can form some different complexes on the apical plasma membrane of renal proximal tubular
cells.
Journal Title
The journal of medical investigation : JMI
ISSN
13431420
NCID
AA11166929
Volume
58
Issue
1-2
Start Page
140
End Page
147
Sort Key
140
Published Date
2011-02
Remark
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
FullText File
language
eng
departments
Oral Sciences
Medical Sciences