| ID | 96861 |
| Author |
Nishimura, Yumiko
Dental Department, Hiroshima University Hospital
Oyama, Yasuo
Graduate School of Integrated Arts and Sciences, The University of Tokushima
KAKEN Search Researchers
|
| Keywords | Lidocaine
Intracellular Zn2+ level
FluoZin-3
|
| Content Type |
Departmental Bulletin Paper
|
| Description | Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+
concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3 fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine on FluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions. 5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-induced augmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions, suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence, suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-group have been proposed to store Zn2+, the decrease in nonprotein thiol content by |
| Journal Title |
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
|
| ISSN | 09146385
|
| NCID | AN10065859
|
| Volume | 26
|
| Issue | 2
|
| Start Page | 7
|
| End Page | 10
|
| Sort Key | 7
|
| Published Date | 2012-04
|
| EDB ID | |
| FullText File | |
| language |
jpn
|
| departments |
Bioscience and Bioindustry
|
