ID 96861
著者
ニシムラ, ユミコ Dental Department, Hiroshima University Hospital
小山, 保夫 Graduate School of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Lidocaine
Intracellular Zn2+ level
FluoZin-3
資料タイプ
紀要論文
抄録
Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+
concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3
fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine on
FluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions.
5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-induced
augmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions,
suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence,
suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-group
have been proposed to store Zn2+, the decrease in nonprotein thiol content by
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
26
2
開始ページ
7
終了ページ
10
並び順
7
発行日
2012-04
EDB ID
フルテキストファイル
言語
jpn
部局
生物資源系